STUDY OF ANTIMICROBIAL ACTIVITY OF "FUZIPAN-DERMA" GEL

Introduction. Providing the population with effective drugs for the treatment of infectious diseases of the skin has an important medical and socio-economic significance. Antibiotic resistance of pathogenic microorganisms can contribute to delaying the pathological process, which is an acute problem not only in dermatology, but also in other spheres of medicine. For the comprehensive treatment of infectious diseases of the skin prescribed medicines for local use. Fusidic acid, which is included in the experimental samples of the gel as an active ingredient, is a natural antibiotic that detects a narrow spectrum of antimicrobial activity. Fusidic acid is primarily active in staphylococcus, including those that are methicillin-resistant. Thus, the use of gel with fusidic acid, which exhibits a pronounced antimicrobial effect on the cells of purulent skin inflammations in acne [1, 2, 3] and used to treat complicated staphylococcus infections also [4, 5]. Materials and methods. Empirical, theoretical and experimental methods, analysis of professional scientific publications, antimicrobial researches and analysis of statistical data have been used. The subject of the study was to determine the antimicrobial activity of 4 experimental samples of the drug "Fuzipan-derma", with different concentrations of the active pharmaceutical ingredient (API), fusidic acid, in its composition [3, 6] and gel-base without fusidic acid as a control. Results and discussion. The results of the studies allow to characterize both the antimicrobial activity of the drug and the speed and completeness of the release of antimicrobial substances from the base, since the zones of growth impairment of microorganisms are formed due to the diffusion of these substances into a dense nutrient medium. In the course of research, one-day suspensions of bacterial microorganisms in a physiological solution were used, and a two-day culture of a yeast-like fungus. The microbial load was 107 microbial cells in 1 ml of nutrient medium. The investigated samples No.1, No.2, No.3 does not show fungicidal activity against yeast-like fungus Candida albicans АТSС 885-653. Sample No.4 (base of gel) does not show antimicrobial activity in relation to the used cultures of bacteria as well as to the fungus of the genus Candida albicans. Samples (No.1, No.2, No.3) exhibit high antimicrobial activity (the diameter of growth impairment zones is 16-25 mm) to gram-positive bacterial cultures Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Bacillus subtiliss. It should be noted that the most susceptible to the action of the samples containing fusidic acid is the culture of Staphylococcus aureus ATCC 25293. With the increase in the concentration of API (fusidic acid) in the gel, antibacterial activity increases: in relation to Staphylococcus aureus ATCC 25293, the diameters of growth impairment zones of the culture are: 25.6±0.5 mm (sample number 1), 33.8±0.4 mm (sample number 2), 35.4±0.5 (sample number 3); to the culture of Staphylococcus epidermidis ATCC 12228: 23.4±0.5, 29.6±0.5, 31.8±0.4 mm respectively 0.5%, 1.0% and 2.0%; to the culture of Streptococcus pyogenes ATCC 12344: 24.2±0.4, 31.6±0.531.6±0.5, 33.6±0.5, respectively, samples with a concentration of fusidic acid of 0.5%, 1.0%, and 2.0%. In relation to the gram-positive spore culture of Bacillus subtilis ATCC 6633, gel samples showed activity at the level of 20.8±0.4, 23.6±0.5, 25.4±0.5, depending on the content of fusidic acid. The research showed that gel samples with fusidic acid content of 3% had a higher antimicrobial activity than 2.0% gel, but in order to minimize the risk of local allergic reactions and reduce the resorptive action of the antibiotic and the development of microbial resistance, it is advisable to select a sample of fusidic acid of 2.0% as a sufficiently effective API concentration. Conclusions. For further studies for development the technology of gel with fusidic acid for the treatment of acne, it is advisable to select sample number 2 with a content of fusidic acid of 2.0%